Lignocellulosic Cellulases

Bioresource engineering science 101 (2010) 87988806 Contents lists available at ScienceDirect Bioresource Technology diary homepage: www.elsevier.com/locate/biortech Characterization of thermostable cellulases produced by Bacillus and Geobacillus examines Gurdeep Rastogi a,1, Aditya Bhalla a, Akash Adhikari a, Kenneth M. Bischoff b, Stephen R. Hughes b, Lew P. Christopher c, Rajesh K. Sani a,* a b c Department of Chemical and Biological Engineering, randomness Dakota School of Mines and Technology, rapid City, SD 57701, ground forces Bioproducts and Biocatalysis explore Unit, National bosom for Agricultural function Research, US Department of Agriculture, Peoria, IL 61604, regular army Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA a r t i c l e i n f o a b s t r a c t The composition of thermophilic (60 °C) mixed cellulose-degrading enrichment culture initiated from conver t samples was examined by constructing a 16S rRNA gene clone library and the presence of sequences related to to Actinobacteria, Bacteroidetes, Chloro?exi, Deinococcus-Thermus, Firmicutes, and Proteobacteria were identi?ed.

Eight secludes capable of degrading cellulose, carboxymethyl cellulose (CMC), or ponderosa hanker away sawdust were identi?ed as belonging to the genera Geobacillus, Thermobacillus, Cohnella, and Thermus. A compost isolate WSUCF1 (Geobacillus sp.) was selected based on its higher growth invest and cellulase natural action compared to others in liquid minimal speciality containing cellulose as a sourc e of carbon and energy. Strain WSUCF1 and a ! previously stray thermophilic cellulose-degrading deep gold mine strain DUSELR13 (Bacillus sp.) were examined for their enzyme properties and kinetics. The optimal pH for carboxymethyl cellulase (CMCase) activity was 5.0 for both isolates. The optimum temperatures for CMCase of WSUCFI and DUSELR13 were 70 and 75 °C, respectively. For CMC, the DUSELR13 and WSUCF1 CMCases had Km values...If you want to get a bounteous essay, order it on our website: OrderCustomPaper.com

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